Interaction ID
ncRI-40000001
Interaction Molecules
4.5S -- rpoD
Interaction Level
RNA-Protein
Interaction Class
binding
Data Source
Literature mining
Tags
Organism
Escherichia coli
Tissue or Cell Type
-
Experiment
Immunoprecipitation experiments;EMSA;SDS-polyacrylamide gel electrophoresis
Description
Following translocation, 4.5S RNA replaces 23S rRNA as a binding site for EF-G. This replacement promotes the dissociation of EF-G from the ribosome. GDP has positive effects on the RNA binding.The conserved decanucleotide sequence (5-GAAGCAGCCA-3) of 4.5S RNA, competed with the 23S rRNA region defining the EF-G-binding site.
Alias
stpA;tufA;rpsA;tolC;tsp;yfbG;ihfA;sspB;marA;ffh;fusA;tufB;hupA;rnr;clpX;csrA;rpoC;livJ;fur;ftsY;soxS;leu0;uvrY;rnpA;ompR;hupB;ihfB;htrA;RelE
Class
protein
UniProt id
Description
Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth. Preferentially transcribes genes associated with fast growth, such as ribosomal operons, other protein-synthesis related genes, rRNA- and tRNA-encoding genes and prfB.
| Pubmed ID | 1281314 | Journal | Nucleic acids research |
|---|---|---|---|
| Title | Evolutionary conserved nucleotides within the E.coli 4.5S RNA are required for association with P48 in vitro and for optimal function in vivo. | ||
| Author | Wood H, Luirink J, Tollervey D | ||
| Pubmed ID | 8107852 | Journal | Nature |
|---|---|---|---|
| Title | Interaction of E. coli Ffh/4.5S ribonucleoprotein and FtsY mimics that of mammalian signal recognition particle and its receptor. | ||
| Author | Miller JD, Bernstein HD, Walter P | ||
| Pubmed ID | 8662727 | Journal | The Journal of biological chemistry |
|---|---|---|---|
| Title | Identification of protein synthesis elongation factor G as a 4.5 S RNA-binding protein in Escherichia coli. | ||
| Author | Shibata T, Fujii Y, Nakamura Y, Nakamura K, Yamane K | ||